|
KMID : 1216720100020010055
|
|
Korean Journal of Gynecologic Endocrinology
2010 Volume.2 No. 1 p.55 ~ p.62
|
|
|
The Effect of Pomegranate on Bone Histomorphometry in Ovariectomized Rat and on Proliferation and Differentiation of Human Mesenchymal Stem Cell in Vitro
|
|
Park Hyun-Tae
Ahn Ki-Hoon
Yi Kyong-Wook
Shin Jung-Ho
Hur Jun-Young
Kim Young-Tae
Kim Sun-Haeng
Lee Kyu-Wan
Kim Seung-Hee
Bae Chun-Sik
Kim Jung-Min
Kim Tak
|
|
|
Abstract
|
|
|
|
Objectives: To determine whether or not pomegranate extract can affect bone histomorphometry in ovariectomized rats and proliferation and differentiation of human mesenchymal stem cell Methods: Human mesehcymal stem cells were cultured in vitro. Proliferation assay and Alkaline phosphatase activity were measured. Seventy-nine female Sprague-Dawley rats were used: A, no intervention; B, sham operation and distilled water; C, ovariectomy and distilled water; D, ovariectomy and 10% dilute pomegranate; E, ovariectomy and 20% pomegranate; and F, ovariectomy and 40% pomegranate. The study samples were obtained 4 weeks later. The following parameters were investigated for analyses: 24-hour food intake, body weight, bone histomorphometry, and serum bone turnover markers Results: pomegranate extract (1/1000 dilution) significantly increase the growth of human mesenchymal stem cell. However, pomegranate extract did not increased Alkaline phosphatase activity. Bone volume was decreased after ovariectomy and the 40% pomegranate extract had a lower trabecular separation. There were lowering effect in serum phosphate. Conclusion: Pomegranate extract has an beneficial effect on bone histomorphometry in ovariectomized rat and human mesenchymal stem cell function.
|
|
|
KEYWORD
|
|
|
Pomegranate, Bone morphometry, Human mesenchymal stem cell
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|